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引用本文:   侯壯豪, 詹柳娟, 欒謀君, 田雙雙, 戴夢杰, 黃光明. 單細胞代謝物的質譜分析及其在單個神經元分析中的應用. 分析化學, 2019, 47(10): 1601-1611. doi:  10.19756/j.issn.0253-3820.191459 [復制]

Citation:   HOU Zhuang-Hao , ZHAN Liu-Juan , LUAN Mou-Jun , TIAN Shuang-Shuang , DAI Meng-Jie , HUANG Guang-Ming . Metabolites Analysis of Single Cell by Mass Spectrometry and Application in Single Neuron Metabolites Analysis. Chinese Journal of Analytical Chemistry, 2019, 47(10): 1601-1611. doi: 10.19756/j.issn.0253-3820.191459 [復制]

單細胞代謝物的質譜分析及其在單個神經元分析中的應用

通訊作者:  黃光明, [email protected]

收稿日期: 2019-07-29

基金項目: 本文系國家自然基金項目(Nos.21775143,21475121)、合肥物質科學技術中心創新項目培育基金項目(No.2017FXCX003)和中央高校基本科研業務費專項資金(No.WK6030000116)資助

Metabolites Analysis of Single Cell by Mass Spectrometry and Application in Single Neuron Metabolites Analysis

Corresponding author:  HUANG Guang-Ming , [email protected]

Received Date:  2019-07-29

Fund Project:  This work was supported by the National Natural Science Foundation of China (Nos. 21775143, 21475121), the Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology (No. 2017FXCX003) and the Fundamental Research Funds for the Central Universities (No. WK6030000116).

針對單細胞的代謝分析具有重要的研究價值,發展對單個神經元內的代謝物的分析技術(單個神經元分析技術)更是研究大腦的基礎。由于代謝物分子種類眾多,濃度差異巨大,目前單細胞代謝分析方法主要是基于質譜技術發展起來的。利用質譜從單個神經元層面對代謝物開展研究,有助于揭示神經元的異質性,為探索神經元工作機制、研究神經元代謝活動以及針對重大腦疾病開發新療法提供新的研究工具。本文對近年來對單細胞代謝物的質譜分析新方法進行了系統評述,從離體培養的神經元、新鮮分離的神經元和組織原位的神經元等方面介紹了單細胞質譜分析技術在單個神經元分析中的應用,并從質譜儀器技術的開發、新分析方法的發展和生物研究應用3個方面對單個神經元的質譜代謝分析的發展前景進行了展望。

關鍵詞:   單個神經元分析, 代謝物分析, 單細胞質譜, 評述
Key words:   Single neuron analysis, Metabolites analysis, Single cell mass spectrometry, Review
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目錄

單細胞代謝物的質譜分析及其在單個神經元分析中的應用

侯壯豪, 詹柳娟, 欒謀君, 田雙雙, 戴夢杰, 黃光明

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