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引用本文:   張帥, 馮濤濤, 張麗, 張美寧. 活體原位電化學檢測鼠腦內的過氧化氫和多巴胺. 分析化學, 2019, 47(10): 1664-1670. doi:  10.19756/j.issn.0253-3820.191454 [復制]

Citation:   ZHANG Shuai , FENG Tao-Tao , ZHANG Li , ZHANG Mei-Ning . In Vivo Electrochemical Detection of Hydrogen Peroxide and Dopamine. Chinese Journal of Analytical Chemistry, 2019, 47(10): 1664-1670. doi: 10.19756/j.issn.0253-3820.191454 [復制]

活體原位電化學檢測鼠腦內的過氧化氫和多巴胺

通訊作者:  張美寧, [email protected]

收稿日期: 2019-07-28

基金項目: 本文系國家自然科學基金項目(No.21874152)資助

In Vivo Electrochemical Detection of Hydrogen Peroxide and Dopamine

Corresponding author:  ZHANG Mei-Ning , [email protected]

Received Date:  2019-07-28

Fund Project:  This work was supported by the National Natural Science Foundation of China (No. 21874152).

在腦神經系統中,過氧化氫(H2O2)和多巴胺(DA)的同時分析具有重要的意義,但二者的同時實時分析具有很大的挑戰。本研究制備了可用于二者同時電化學分析的環盤微電極,中間的碳纖維盤(CFdisk)電極通過電化學沉積選擇性地修飾普魯士藍(PB),并電聚合聚3,4-乙烯二氧噻吩(PEDOT)保護PB,檢測H2O2,金環(Auring)電極檢測DA。研究結果表明,此電極對H2O2的線性檢測范圍為1~29 μmol/L,檢出限為0.4 μmol/L;對DA的線性檢測范圍為0.5~25 μmol/L,檢出限為0.18 μmol/L,兩電極之間未交叉干擾。采用此電極檢測了大鼠腦內的DA和H2O2濃度的變化。本研究為有關H2O2和DA的神經生理和病理的研究提供了新的方法。

關鍵詞:   過氧化氫, 多巴胺, 活體, 微電極, 電化學分析
Key words:   Hydrogen peroxide, Dopamine, In vivo, Microelectrodes, Electrochemical analysis
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目錄

活體原位電化學檢測鼠腦內的過氧化氫和多巴胺

張帥, 馮濤濤, 張麗, 張美寧

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